Genotyping & Gene Expression Solutions
Your Industry, Our Focus
Gene Expression Analysis & Genotyping
At Eurofins Genomics, we offer genotyping and gene expression services tailored to meet your research needs.
Our services encompass DNA typing or genotyping using PCR/Real-time PCR methods with common dominant and co-dominant markers, as well as the Sanger-based SNP detection method.
For gene expression studies, we provide a one-stop solution using SYBR Green as well the TaqMan chemistry (We offer >20 different modified oligos from our Bangalore in-house facility).
Why Choose Eurofins Genomics for Your Genotyping & Gene Expression Needs?
- Comprehensive Services
We offer a full range of genotyping and gene expression solutions, ensuring all your research needs are met in one place.
- Fast Turnaround
Efficient processes and in-house capabilities mean you receive your results quickly.
- Customer Support
Our team of experts is always available to provide support and guidance throughout your project.
Our Services
- Fragment Analysis/Microsatellite Genotyping (ABI3730XL):
- We perform fragment analysis on DNA fragments with fluorescent labels using PCR amplification with labelled primers.
- Compatible with various dye sets: 6-FAM, VIC, NED, PET, LIZ (ABI Dye Set G5, DS-33) based on the custom requirement.
- Samples are set up in approved 96-well plates and can be less (please coordinate with our regional sales team).
Sample Submission Guidelines
- 20µl gDNA @ 100ng/µl
- Unpurified or purified tagged/labelled PCR product
- Provide sample names according to the plate format. For sizing information, include labelling and product size details.
- Genotyping by PCR
- We use traditional PCR-based markers like RAPD, ISSR, and SSR to determine polymorphism among samples.
- This service is simple, robust, cost-effective, and ideal for genetic screening programs that require bulk sample screening.
- In-house primer synthesis ensures convenience and faster results.
Sample Submission Guidelines
- 500 mg to 1g tissues
- gDNA, Vol. 50µl (according to the number of markers), Conc. 30ng/µl
- Primers (optional)
- SNP Genotyping:
- SNPs (single nucleotide polymorphisms) are key genetic variations that contribute to phenotypic diversity.
- We offer deep genome analysis and genotype-phenotype connection studies using SNPShot Kit or General Sanger sequencing method.
Sample Submission Guideline:
- 20µl gDNA @ 100ng/µl
Frequently Asked Questions ( FAQ's )
What is genotyping by PCR?
Genotyping by PCR involves using polymerase chain reaction (PCR) to amplify specific hot-spot region of DNA, which are then analysed to identify genetic variations.
What is the difference between dominant and co-dominant markers?
Dominant markers only reveal the presence or absence of a trait, while co-dominant markers show both alleles, providing more detailed genetic information.
How does fragment analysis work?
Fragment analysis involves labelling DNA fragments with fluorescent dyes and separating them by size using capillary electrophoresis, allowing for the detection of genetic variations.
What are SNPs and why are they important?
SNPs are single nucleotide polymorphisms, which are the most common type of genetic variation. They play a crucial role in determining individual traits and susceptibility to diseases.
How can I submit my samples for analysis?
Samples can be submitted according to the specific guidelines for each service. Detailed instructions and order forms are provided to ensure accurate and efficient processing.
Choose Eurofins for reliable, high-quality genotyping and gene expression solutions tailored to advance your research and achieve your scientific goals.
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