SYBR Green Chemistry
Your Industry, Our Focus
At Eurofins Genomics, we provide comprehensive gene expression study services using SYBR Green Chemistry with optimization of your target genes. Researchers benefit from our flexibility in sample handling and data generation while ensuring the highest accuracy in results.
Service Overview
Our SYBR Green Chemistry gene expression study includes a complete experimental setup tailored to your gene of interest. We handle the entire process from RNA isolation to data analysis, ensuring reliable and reproducible results.
Here's what our service offers
- RNA Isolation and Purification
We extract and purify RNA from various sample types, including tissues, bacterial cultures, and other biological species.
- RNA Quantification:
We quantify RNA using both denatured gel and Nano Drop spectrophotometry, ensuring accurate RNA concentration for downstream applications.
cDNA Synthesis
RNA is converted into complementary DNA (cDNA) using our optimized protocols.
Primer/Probe Synthesis
Gene-specific primers or probes are synthesized to target your gene of interest with high specificity (client may share the optimized gene primers and probes sequences if validated.
Primer Efficiency Calculation
Primers efficiency will be optimized with the gradient.
Housekeeping Gene Selection
The client needs to provide the house gene information as well as the probes and primers sequences.
Quantification
Quantification of gene expression is conducted, depending on your experimental requirements.
Sample Requirements
We accept a wide range of sample types, including:
- Isolated pure DNA/RNA
- Pure bacterial cultures (slants, plates, broth), and tissue samples (plant, animal, insect)
- Gene sequences provided in FASTA format
- Primers sequences to target the gene
Deliverables
Our service includes
- A comprehensive report detailing absolute or relative gene expression results.
- Raw data, including Ct values for each sample.
Why Choose Eurofins Genomics?
Choosing our SYBR Green Chemistry gene expression study service ensures that you receive high-quality, accurate, and reproducible data. Our experts are equipped to handle various sample types and experimental designs, providing you with the flexibility and support needed to achieve your research goals.
Frequently Asked Questions (FAQs)
What is SYBR Green Chemistry, and how does it work in gene expression analysis?
SYBR Green Chemistry is used in quantitative PCR (qPCR) to measure gene expression levels. SYBR Green dye binds to double-stranded DNA, and its fluorescence increases upon binding. During PCR amplification, the fluorescence is monitored in real-time, allowing for the quantification of the DNA and, thus, the gene expression levels.
What are the advantages of using SYBR Green Chemistry over other methods?
SYBR Green Chemistry is cost-effective, simple to use, and requires only a single dye for detection, making it suitable for a broad range of applications. It is highly sensitive and can detect low levels of gene expression, and the lack of a probe design simplifies the process compared to methods like TaqMan.
What types of samples can be submitted for SYBR Green Chemistry gene expression analysis?
We accept isolated pure DNA/RNA, bacterial cultures, tissue samples (plant, animal, insect), and gene information in FASTA format.
Can SYBR Green Chemistry be used for multiplexing (analyzing multiple genes in one reaction)?
SYBR Green Chemistry is not ideal for multiplexing because it uses a single dye that binds to all double-stranded DNA, making it difficult to distinguish between different targets. For multiplexing, probe-based chemistries like TaqMan are preferred, as they allow for the simultaneous detection of multiple genes in a single reaction.
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