Taq Man Chemistry
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Taqman chemistry is now one of the gold standard method for quantification of target genes in diagnostic setup. This chemistry can further use for absolute and relative quantification based on your experimental finding. If your research need study multiplexed gene expression in one or more sample with high accuracy and low noise ratio, then please avail our TaqMan chemistry for your gene expression study.
We are providing one step solution of, in house primer synthesis, and high quality data generation.
Sample submission
Under RT-PCR service we provide:
- Isolation and purification of RNA from sample/ Tissue
- Quantification of RNA on denatured gel as well as Nano-drop
- RNA to cDNA conversation
- Gene specific primers/ probe synthesis
- Primer efficiency calculation
- Housekeeping gene synthesis (depending upon the type of sample)
- Relative quantification (by using data from triplicate reaction) or absolute quantification.
Samples
- Isolated pure DNA/RNA
- Pure bacterial culture on slants/plate/broth, tissue (Plant/animal/insect)
- Gene information in FASTA format
Deliverable
- Absolute or Relative Gene expression compiled report
- Raw data Ct value.