Sanger Sequencing

Eurofins offers Sanger Sequencing Service using high throughput ABI3730 Xl platform with BigDye V3.1 terminator technology. Sanger sequencing is gold standard technique for short read sequencing to harness the genomic information or short gene information in given biological samples. This is one of the cost effective sequencing platform still used for various research work for confirmation of gene sequence.

Eurofins is one of the best market leader to cater various sequencing services on ABI capillary sequencers. We accept various kinds of samples ranging from different types of direct PCR product, gDNA, Non-pathogenic microbial culture, purified DNA plasmid, gene extracted products, Tissue, DNA/cDNA, PCR product and nested PCR products.

To hassle free order entry to bring more transparency in system, we have unified samples submission form available on line for avail our sequencing services. All samples are received by our trained tab technicians and are verified all the correct information to avoid any pre and post error.

Once physical QC check through, samples are passes to our QC division to check quality and right concentration to impurities, product size. If sample fail to pass our QC criteria, or If any ambiguity with samples quality, our expert sequencing team will communicate with you via phone or mail.

Based on the nature of the sample, Eurofins has divided Sanger sequencing in categorized in 6 classes

• Single Pass Seq: Under this service, we can provide DNA/ plasmid isolation to single pass sequence up to 800 bases.   You can also provide us tissue samples, crude DNA, plasmid or PCR products.
• Bi-directional Seq: Under this service we can provide DNA/ plasmid isolation to single pass sequence up to 1500 bases.   You can also provide us tissue samples, crude DNA, plasmid or PCR products.
• Culture Seq: We are providing high-quality DNA isolation from pure microbial culture, generating amplicons up to 3kb and providing amplicon sizing report.
• Primer Walking Single Direction: This service starts with our standard primers or your specific primers to sequence the first 750 - 1000 bases of the DNA strand.
• Primer Walking Bi-Direction: Primer walking bi-direction:  This service starts with our standard primers or your specific primers to sequence the first 750 - 1000 bases of the DNA strand.
• Applied Genomics Services: We have several applied services which help the industrial as well as academic researches to meet their goals. Under this service we have 3 different services.