Unlock the complete sequence of your DNA strand with our efficient Single Strand Primer Walking service. Whether using our standard primers or your custom-designed primers, this service ensures precise and comprehensive sequencing of the DNA strand in stages, starting with the first 750-1000 bases. As the sequencing progresses, new primers are synthesized based on the results, allowing us to "walk" along the DNA strand until the entire sequence is fully determined. With a final data accuracy of over 99.90%, our service delivers reliable and high-quality results for your research needs.

Key Service Features

• Initial Primers: Use of standard or customer-specific primers to initiate the sequencing process.
• Step-by-Step Progress: Sequential synthesis of new primers based on the obtained sequence, enabling accurate extension of the sequence until completion.
• Guaranteed Accuracy: Final sequencing accuracy exceeds 99.90%, ensuring trustworthy data for your analysis.

Sample Requirements

• Volume & Concentration: 50µl with a concentration of 200ng/µl of plasmid sample.
• Primer Information: Forward and reverse primer sequences must be provided.

Deliverables

• Consensus Sequence: Provided as a FASTA file.
• Additional Files: .abi files and chromatograms in PDF format for all reactions.