Primer Walking Single Direction

This service starts with our standard primers or your specific primers to sequence the first 750 - 1000 bases of the DNA strand. Based on the resulting sequence data, we define and synthesize new primers which are used to sequence further bases thus “walking” along the sequence until the entire strand is deciphered. A final data accuracy of > 99.90% is guaranteed with our single strand primer walking.

Sample submission:

• 50µl Vol. and 200ng/µl of plasmid sample
• primer sequences of forward & reverse primers

Deliverables:

• Consensus sequence as FASTA file
• .abi file, chromatogram in pdf file for all reaction