Eurofins Genomics India offers a wide range of comprehensive genome sequencing services, from prokaryotes to eukaryotes. Our solutions provide complete and accurate sequencing of entire genomes, enhancing the understanding of genomic structural variations and species diversity.

We offer end-to-end services including sample preparation, sequencing, and bioinformatic analysis, tailored to provide the optimum sequencing strategy.

We Perform Genome Sequencing on Advanced Platforms of Illumina (NovaSeq X, NextSeq500 and MiSeq) and Nanopore.

Services

     I. De Novo Genome

• Sequencing of unknown genomes (no reference sequence available in public database)
• Hybrid Assembly of short and long reads using Illumina and nanopore.

  II. Re-sequencing

• Ideal when a good reference sequence is available.
• Mapping of reads to the reference sequence, optional variance analysis.

Bacterial and Fungal Genome Sequencing

We utilize a strategic combination of sequencing and scaffolding to achieve high-quality de novo sequencing of small genomes, drastically reducing post-sequencing efforts.

Methodology

• We accept NGS grade high-quality gDNA, pure microbial cultures, and fungal mycelia.
• Sample quality is ensured through thorough our QC steps including agarose gel electrophoresis, NanoDrop/Qubit Fluorometer analysis, and Agilent 4200 Tape Station.
• Library preparation involves the combination of paired-end shotgun libraries.
• Sequencing is performed on advanced platforms such as Illumina NextSeq500 and NovaSeq X, ensuring high throughput and accuracy.

Deliverables

• Raw data in FASTQ files.
• Comprehensive compiled reports including genome assembly metrics, annotation summaries, and many other analysis, please refer bioinformatics page.
• Data sets shared via secure links, Pen Drive, or Hard Disk.

Eukaryotic Genome Sequencing

Eurofins Genomics offers de novo sequencing for large eukaryotic genomes, leveraging advanced sequencing platforms and processes to manage complex, repeat-rich genomes.

Methodology

• Accepts high-quality gDNA and tissue samples.
• Ensures RNA-free NGS grade gDNA isolation.
• Prepares libraries combining paired-end shotgun with insert sizes tailored to genome complexity. Also for hybrid assembly can generate long read libraries.
• Utilizes Illumina platforms for short reads (NextSeq500, NovaSeq) and nanopore for long reads sequencing.

Deliverables

• Raw data in FASTQ files.
• Detailed reports including genome coverage statistics, quality metrics for prepared libraries, and comprehensive analysis results.
• Data sets provided in accessible formats for further bioinformatic exploration.

Plasmids, and Virus Genome Sequencing

Eurofins Genomics India provides advanced sequencing solutions for small genomes and large insert constructs, using long-read technology to achieve high-quality de novo sequencing.

Methodology

• Accepts high-quality gDNA, fosmids, plasmids, and viral nucleic acids.
• Rigorous quality control ensures integrity of sequencing data.
• Library preparation includes specialized kits like Nextera XT and TruSeq Nano DNA, tailored for various genome types.
• Sequencing is performed on platforms such as Illumina MiSeq, producing long reads for enhanced contig assembly.

Deliverables

• Raw data in FASTQ files.
• Comprehensive reports encompassing detailed genomic coverage, alignment statistics, and structural variant detection.
• Results provided in user-friendly formats for immediate interpretation and downstream analysis.

Re-sequencing of Genomes

Re-sequence whole genomes to identify genetic variations compared to a reference genome. Our experts use platforms like Illumina NovaSeq, NextSeq 500, and MiSeq to deliver high-quality results.

Methodology

• Accepts high-quality gDNA and tissue samples.
• Comprehensive quality control checks ensure data accuracy.
• Library preparation includes fragmenting genomic DNA, adapter ligation, and optional preparation of multiple shotgun libraries per sample.
• Utilizes advanced sequencing technologies and library types for optimal genome coverage.

Deliverables

• Raw data in FASTQ files.
• Detailed reports including SNP and InDel analysis, coverage depth metrics, and genome comparison summaries.
• Data sets formatted for immediate use in downstream bioinformatics analyses.

WGS Metagenome Sequencing

Metagenomics sequencing aims to explore genes from microbial communities, generating detailed metabolic and functional profiles.

Methodology

• Accepts meta-genomic DNA, soil, sludge, water, and other environmental samples.
• Ensures high-quality DNA extraction and purification.
• Library preparation tailored to sample type, utilizing kits like TruSeq Nano DNA or Nextera XT.
• Sequencing performed on Illumina platforms (NextSeq500,  NovaSeq) to achieve comprehensive coverage and resolution.

Deliverables

• Raw data in FASTQ files.
• Comprehensive reports detailing taxonomic classification, functional gene annotation, and diversity indices.
• Data sets provided in formats suitable for ecological and functional genomic analyses.

Why Choose Eurofins Genomics for Your Genome Sequencing Needs?

1. State-of-the-Art Technology: Utilizing the latest platforms like NextSeq500, NovaSeq6000, Nanopore.
2. Comprehensive Solutions: Offering end-to-end services from sample preparation to bioinformatic analysis.
3. Expertise: Extensive experience in sequencing a wide range of organisms.
4. Quality Assurance: Rigorous quality control processes ensure reliable and accurate results.