Dark Quencher Modifications
Your Industry, Our Focus
Enhance the sensitivity and precision of your molecular assays with our Dark Quencher Modification Services. Dark quenchers provide highly efficient fluorescence suppression without emitting light themselves, making them ideal for a range of applications including quantitative real-time PCR, FRET probes, and Molecular Beacons.
Our selection of dark quenchers covers a wide spectral range, allowing for optimal quenching across different dye emissions.
Available Dark Quenchers
Dabcyl (DAB)
Dabcyl is a non-fluorescent quencher that effectively suppresses fluorescence in the blue to green emission spectrum. Ideal for fluorophores with emission wavelengths between 400-550 nm, it is not recommended for use with dyes emitting above 480 nm.
- Quenching Range: 400-550 nm
- Max. Absorbance: 453 nm
- Molar Extinction Coefficient: 32,000 M−1 cm−1
- Molecular Weight: 399.2 g/mol
Dabcyl-dT (DABdT)
An internal quencher variant of Dabcyl, Dabcyl-dT efficiently quenches dyes in the blue to green emission range. It can be used in combination with 3' quenchers for enhanced quenching efficiency.
- Quenching Range: 400-550 nm
- Max. Absorbance: 453 nm
- Molar Extinction Coefficient: 32,000 M−1 cm−1
- Molecular Weight: 399.2 g/mol
Eclipse (Eclip)
Eclipse is a non-fluorescent quencher from Epoch Biosciences, offering broad quenching from 390 to 625 nm. It is highly efficient for use with Dual-Labeled Probes and Molecular Beacons.
- Quenching Range: 390-625 nm
- Max. Absorbance: 522 nm
- Molar Extinction Coefficient: 53,000 M−1 cm−1
- Molecular Weight: 473.9 g/mol
BHQ1 (Black Hole Quencher 1)
BHQ1 is a powerful quencher for green to dark yellow emission spectra, commonly used in FRET probes, Molecular Beacons and TaqMan chemistry.
- Quenching Range: 480-580 nm
- Max. Absorbance: 534 nm
- Molar Extinction Coefficient: 34,000 M−1 cm−1
- Molecular Weight: 491.5 g/mol
BHQ2 (Black Hole Quencher 2)
BHQ2 extends quenching efficiency into the dark green to orange emission spectrum, making it suitable for use in Dual-Labeled Probes with 5' fluorophores.
- Quenching Range: 520-650 nm
- Max. Absorbance: 544 nm
- Molar Extinction Coefficient: 91,000 M−1 cm−1
- Molecular Weight: 493.5 g/mol
BBQ650 (BlackBerry Quencher 650)
BBQ650 is ideal for long-wavelength dyes such as Cy5 and Cy5.5, offering excellent quenching for dyes emitting in the red to near-infrared spectrum.
- Quenching Range: 550-750 nm
- Max. Absorbance: 650 nm
- Molar Extinction Coefficient: 41,000 M−1 cm−1
- Molecular Weight: 575.6 g/mol
Product Specifications
- Purity: All quenchers are HPLC purified for >80% purity.
- Synthesis Scale: Customizable from 0.05 to 1.0 µmol.
- Sequence Length: Available for oligos ranging from 10 to 80 bases.
- Turnaround Time: Typical delivery within 3-4 weeks.
- Quality Control: Each oligo is rigorously tested using OD measurement and MALDI-TOF mass spectrometry to ensure precision and purity.
Frequently Asked Questions (FAQs)
What are dark quenchers used for?
Dark quenchers are used to suppress fluorescence without emitting light themselves, making them highly effective for applications such as real-time PCR, FRET, and Molecular Beacons, where precise fluorescence detection is crucial.
How do I choose the right dark quencher for my experiment?
The choice of dark quencher depends on the emission wavelength of your fluorophore. For example:
- Dabcyl is ideal for blue to green fluorophores (400-550 nm).
- BHQ1 is suitable for fluorophores emitting in the green to yellow range (480-580 nm).
- BBQ650 is best for red to near-infrared fluorophores (550-750 nm).
Can I use more than one dark quencher in a single probe?
Yes, multiple dark quenchers can be used in a single probe for enhanced quenching, such as combining internal quenchers like Dabcyl-dT with 3' quenchers.
What are the benefits of using dark quenchers in qPCR?
Dark quenchers improve the sensitivity and accuracy of qPCR by eliminating background fluorescence, ensuring that the fluorescent signal is only generated when the target sequence is amplified.
What quality control measures do you implement for dark quencher-modified oligos?
All dark quencher-modified oligos undergo HPLC purification and are subject to stringent quality control checks, including OD measurement and mass spectrometry (MALDI-TOF), to guarantee high purity and precise modification.
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