DNA Oligo Modification
Your Industry, Our Focus
Benefit from 20 Years of Experience in DNA Oligo Modification
Our DNA modification centre, backed by two decades of expertise, offers a wide range of common and alternative modifications to suit your unique applications.
We provide various synthesis scales and purification options to meet your specific needs. We offer a broad selection of dye combinations to ensure timely solutions for all your needs.
Our offerings include
- Common Reporter Dyes: FAM, HEX, TET, JOE, ROX, TexRED, TAMRA, Biotin, Amino C6 & C3 linker, Phosphorylation etc.
- Cyanine Dyes: CY5, CY5.5, CY3
- Quencher: BHQ1, BHQ2, BBQ650, MGB, TAMRA
- ATTO and Dyomics Dyes
- Molecular Probes Dyes: Alexa Fluor, Bodipy dyes
Choosing the Right Fluorescent Dye
Selecting the appropriate fluorescent dye for your assay can be challenging. Here are some key criteria to consider:
Absorption and Emission Spectra
The absorbance of a dye indicates how much visible or UV light it absorbs, while the emission value shows the wavelength at which it emits light.
These properties are critical for matching dyes with your instrument’s features, such as excitation sources and optical filters.
Stokes Shift
The Stokes shift is the difference in energy between the excitation and emission maxima. A large Stokes shift is often preferable for separating exciting light and fluorescence emission using optical filters.
Extinction Coefficient
This value measures a dye's ability to absorb light, affecting the intensity of the emitted light.
pH Sensitivity
Dye stability can vary with pH levels. Some dyes degrade in alkaline conditions above pH 9, while others remain stable across a wide pH range.
Stability to Photobleaching
Photobleaching refers to the destruction of a fluorophore due to light exposure. Consider the photostability of a dye based on your application; for example, it is more critical for fluorescence microscopy than for DNA sequencing.
Fluorescence Quantum Yield
The fluorescence quantum yield indicates the efficiency of the fluorescence process, defined as the ratio of emitted to absorbed photons. This yield is influenced by factors like pH, solution type, concentration, and temperature.
Frequently Asked Questions ( FAQ's )
What are the absorption and emission spectra of fluorescent dyes?
The absorption spectrum indicates how much light a dye absorbs, while the emission spectrum shows the wavelength at which it emits light. These values are crucial for selecting dyes that match your instrument's capabilities and your specific assay needs.
Why is photostability important in fluorescence microscopy?
Photostability refers to a dye's resistance to photobleaching, which can affect fluorescence intensity and lead to erroneous results. In applications like fluorescence microscopy, high photostability is essential to maintain accurate observations over time.
What factors influence the fluorescence quantum yield of a dye?
The fluorescence quantum yield depends on the dye's microenvironment, including pH, solution type, concentration, and temperature.
Contact us today to discuss your DNA Oligo modification needs.
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