Oligonucleotide Purification
Your Industry, Our Focus
Custom DNA Oligos
Tailored to your specifications, available as Salt-Free or purified via our proprietary HPSF method or HPLC.
High Purity Salt-Free (HPSF) Purification
- Proprietary reverse-phase cartridge purification
- Removes Salt & chemicals carryover
- Free from disturbing salts which may inhibit PCR
High-Performance Liquid Chromatography (HPLC) Purification
- Reverse phase chromatography purification
- Eliminates truncated and n-1 sequences.
- Purity may achieved >80%
Why Choose Eurofins for Oligo Purification?
- Advanced purification techniques ensuring high-quality oligos
- Proprietary methods for optimal results
- Flexible customization options to meet specific research needs
Frequently Asked Questions (FAQs)
What is HPSF purification?
HPSF (High Purity Salt-Free) purification uses proprietary reverse-phase cartridge technology to remove chemicals and salts to get the highly purified oligos.
How does HPLC purification work?
HPLC (High-Performance Liquid Chromatography) purification involves reverse phase chromatography using high pressure with the Oilgo-specific columns like C18 reverse phase columns to eliminate truncated sequences, achieving a purity level >80%.
What are the benefits of using Eurofins' oligo purification services?
HPLC is a choice of methods to get highly purified oligo which is > 80%. The HPLC method allows us to remove n-1 truncated sequences which may inhibit the high-end PCR.
How do I choose between HPSF and HPLC purification?
HPSF is ideal for the oligo bases length <30 bases which primarily helps to remove all the inhibitory salts to get specific PCR amplicon, while HPLC is suitable for >35 bases wherein higher purity is required (>80%) which helps to remove the n-1 and the truncated sequences. The choice depends on your specific research requirements
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