Frequently Asked Questions (FAQs)

What types of modifications can I add to my RNA oligos?

We offer a wide range of RNA modifications, including fluorescent dyes, non-fluorescent functional groups (e.g., amino and biotin), spacers, linkers, sugar and base modifications, and dark quenchers. These modifications can be placed at the 5’, internal, or 3’ positions depending on your experimental needs.

How do I choose the right modification for my application?

The modification you choose depends on your experiment's purpose. For example:

• Fluorescent dyes are ideal for visualization and tracking.
• Amino or biotin modifications allow for further conjugation or binding.
• Dark quenchers are perfect for FRET and qPCR applications.

If you're unsure, our technical team can help you select the best modification for your specific requirements.

What is the typical turnaround time for modified RNA oligos?

Our standard turnaround time for modified RNA oligos is 3-4 weeks, depending on the complexity and number of modifications required.

What is the maximum sequence length you can synthesize with modifications?

We can synthesize RNA oligos with sequence lengths ranging from 10 to 80 bases, depending on the modifications. Some modifications may limit the overall length, so please contact us if your project requires longer sequences.

How are modified RNA oligos purified ?

All our modified RNA oligos undergo HPLC purification to ensure a minimum purity of >80%, measured by Capillary Gel Electrophoresis (CGE). This guarantees high-quality products for accurate experimental results.

Can I combine multiple modifications in one RNA oligo?

Yes, you can combine different modifications on a single RNA oligo, such as adding a fluorescent dye at the 5' end and a quencher at the 3' end. Let us know your requirements, and we can customize the synthesis accordingly.