qPCR probes

qPCR probes are known as Fluorescent probes. FRET assays effectiveness of qPCR relies on highly specific and efficient amplification of the target sequence and the inclusion of fluorescent reporter molecules that enable the quantification of PCR products in real time. The use of a PCR primer specifically designed and validated for qPCR assays with your target of interest is highly recommended Fluorescent reporter molecules can be DNA-binding dyes such as SYBR® Green or fluorescently labeled PCR primers or probes. There are many different kinds of fluorescent dyes, PCR primers, and probes available for different applications. This section describes the various types of fluorescent dyes, PCR primers, and probes, and their specific uses in real-time PCR experiments.

Excellence in probe-based real-time PCR which can be utilize for various application including Gene expression, target identification, SNP Genotyping or mutation detection

Are you performing gene expression analysis, SNP genotyping or mutation detection via real-time PCR?

Increase the efficiency, sensitivity and specificity of your real-time PCR results by using hybridization probes.

Select the qPCR Probe you need for your application, method and technology:

Eurofins offer wide range of qPCR probes for molecular biology applications. In qPCR, we have various dyes chemistry including 

• MGB Probe: Short probes that incorporate a minor groove binder (MGB); to be used in 5’ nuclease (TaqMan - MGB) technology for human IVD applications
• Duel Labeled Probes: Hydrolysis probes for TaqMan assays with a variety of dye - quencher combinations compatible with all type of instruments.
• Light Cycler probes: Donor and acceptor probes to be used in FRET (fluorescence resonance energy transfer) assays.
• Molecular Beacons: Hairpin shaped hybridisation probes with an internally quenched fluorophore to detect specific sequences.

Probe based qPCR enables simultaneous detection of multiple targets in a single reaction (multiplex PCR) in contrast to unmodified primers with intercalating dyes.