Light Cycler Probes
Your Industry, Our Focus
Elevate Your Assays with FRET-Based Light Cycler Probes for Sensitive Detection
Eurofins Genomics offers Light Cycler Probes that leverage FRET technology for precise detection and quantification study with real-time PCR. The probes are designed for applications that demand high specificity—such as SNP detection, pathogen quantification, and multiplexing—Light Cycler Probes offer a versatile solution for complex molecular diagnostics.
Why Choose Light Cycler Probes?
Light Cycler Probes are optimized for high-stakes applications, including:
- SNP (Single Nucleotide Polymorphism) detection
- Allele discrimination
- Pathogen detection
- Viral load quantification
- Gene expression analysis
- Gene copy determination
These probes deliver precision through
- Enhanced Specificity: Ensures accuracy even in complex assays
- Improved Thermal Stability: Maintains probe integrity throughout reactions
- Multiplex Compatibility: Supports multiple targets in a single run
- Design Flexibility: Offers simplified solutions for complex or challenging targets
Product Specifications
Our Light Cycler Probes are available with these specifications:
- Final yields: 1, 3, 5, and 10 nmol
- Probe length: 15-40 bases
- Purity: HPLC-purified for optimal results
- Delivery: Lyophilized, in convenient tube format
- Quality Control: Stringent QC by OD measurement and MALDI-TOF MS
- Lead Time: Typical turnaround is 2-3 week
How Light Cycler Probes Work
In FRET-based assays, Light Cycler Probes work in pairs that hybridize closely on target DNA sequences. The donor probe, labelled with a fluorescein dye, transfers energy to the acceptor probe upon excitation. This energy transfer occurs only when the probes are bound in proximity on the target, producing a light signal detectable at specific wavelengths. This signal reflects the presence and amount of the target, allowing for accurate measurement and interpretation.
Frequently Asked Questions (FAQs)
What are Light Cycler Probes best suited for?
Light Cycler Probes used in wide variety of molecular diagnostic applications like SNP detection, pathogen identification, viral load quantification, and gene expression analysis, particularly in multiplex and sensitive assays requiring high specificity.
What is the main advantage of using FRET technology with Light Cycler Probes?
FRET technology enables real-time detection of target sequences by utilizing energy transfer between fluorescent dyes, providing high specificity and sensitivity. This method is especially valuable in assays where precise discrimination of genetic sequences is crucial.
How do Light Cycler Probes ensure specificity in SNP detection?
The FRET-based design of Light Cycler Probes allows them to precisely bind to target DNA, making them highly specific for identifying single nucleotide variations, such as SNPs, by producing a unique fluorescent signal.
Are LightCycler Probes compatible with multiplex analysis?
Yes, LightCycler Probes support multiplexing, allowing multiple target sequences to be detected simultaneously. They are compatible with various dyes and instrument setups, enhancing versatility in complex assays.
How are Light Cycler Probes delivered and what is the expected turnaround time?
Probes are lyophilized and shipped in tube format. Post synthesis, undergo Quality-control through the OD measurement and MALDI-TOF MS to ensure the accurecy, the probes typically have a turnaround time of 2-3 weeks from order to delivery.
Discover how Light Cycler Probes by Eurofins Genomics can streamline and elevate your genetic analysis with unmatched precision and reliability.
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