siMAX siRNA
Your Industry, Our Focus
Eurofins Genomics helps you achieve effective gene silencing with our siMAX siRNA through specific Oligo synthesis strategies, which play an important role in the RNA interference (RNAi) pathway, where it interferes with the expression of specific genes with complementary nucleotide sequences.
Why Choose siMAX siRNA?
- High Purity: Each siMAX siRNA duplex is guaranteed and purified with the HPLC to achieve a purity level of 85% or greater.
- Quality Control: Stringent QC check with the MALDI-TOF of both the sense (passenger) and antisense (guide) strands.
- Affordable Excellence: Offering Economic prices without compromising your lab budget.
Specifications
- Purity: ≥80%
- Quantity: Available with 20 nmoles and 40nmoles of both sense and antisense RNA oligos.
- Format: Delivered as dried, fully annealed duplexes.
- Buffer: siRNA Dilution Buffer included with each shipment.
How to Resuspend siMAX siRNA Duplex
- Preparation: Dilution buffer prepared with RNAse-free solutions under the strict compliance guidelines.
- Rehydration: Add siRNA Dilution Buffer as recomonded.
- Heating: Heat the tube to 90°C for 60-90 seconds.
- Incubation: Incubate at 37°C for at least one hour.
- Storage: Store unused stock solution as detailed in the instructions.
Frequently Asked Questions (FAQ's)
What are the benefits of using siMAX siRNA for gene silencing experiments?
siMAX siRNA offers high fidelity in guide strand delivery, ensuring precise gene silencing. Its high purity and comprehensive quality control make it a reliable choice for consistent results.
Can siMAX siRNA be used for in vivo experiments?
Yes, siMAX siRNA can be used for in vivo experiments, but ensure that you follow appropriate protocols and consult with experts if needed to optimize delivery and efficacy in living organisms.
What is the typical shelf life of siMAX siRNA after resuspension?
The typical shelf life can vary, but properly stored siRNA stock solutions can generally remain stable for several months. Always refer to the specific storage instructions provided with your shipment.
What is the difference between siRNA and microRNA?
siRNA (small interfering RNA) and microRNA (miRNA) both play roles in gene silencing but differ in origin and function. siRNA is often exogenously introduced and perfectly matches its target mRNA, leading to mRNA degradation. miRNA is endogenous, typically imperfectly matches its target, and regulates gene expression by inhibiting translation or causing mRNA decay.
How do I confirm that gene silencing has occurred after using siMAX siRNA?
Confirm gene silencing by measuring the mRNA levels of the target gene using qRT-PCR or by assessing the protein levels using Western blot analysis or ELISA.
Are there any known off-target effects with siMAX siRNA, and how can they be minimized?
Off-target effects can occur when siRNA sequences partially match non-target mRNAs. To minimize this, design siRNA with high specificity, use lower concentrations, and validate results with multiple siRNA sequences targeting different regions of the same gene.
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